PRP Tubes Acd Tubooyinka
Sharaxaad Gaaban:
Xalka xinjirowga lidka ku ah Citrate Dextrose Solution, oo caadi ahaan loo yaqaan ACD-A ama Solution A waa xal aan pyrogenic ahayn, oo nadiif ah.Cunsurkaan waxa loo isticmaalaa sidii xinjirowga lidka ku ah soo saarista Plasma-hodonka leh ee platelet (PRP) oo leh Nidaamyada PRP ee habaynta dhiiga ka baxsan jidhka.
Xalka xinjirowga lidka ku ah Citrate Dextrose Solution, oo caadi ahaan loo yaqaan ACD-A ama Solution A waa xal aan pyrogenic ahayn, oo nadiif ah.Cunsurkaan waxa loo isticmaalaa sidii xinjirowga lidka ku ah soo saarista Plasma-hodonka leh ee platelet (PRP) oo leh Nidaamyada PRP ee habaynta dhiiga ka baxsan jidhka.Dawooyinka xinjirowga lidka ku ah ee ku salaysan citrate waxay isticmaalaan awoodda citrate ion si ay u chelate calcium ionized taas oo ku jirta dhiigga si looga hortago xinjirowga dhiigga oo ay sameeyaan kalsiyum-citrate oo aan ionized ahayn.
Alaabta kaliya ee xinjirowga lidka ku ah ee Maamulka Cunnada & Dawooyinka ee Mareykanka ay ansixiyeen in loo isticmaalo diyaarinta PRP ee Nidaamyada PRP ee kala duwan waa ACD-A.Marka loo eego cilmi-baaris lagu sameeyay 2016 PRP oo laga helay dawooyinka xinjirowga lidka ku ah ee kala duwan iyo saameyntooda habdhaqanka unugyada stromal mesenchymal ee vitro iyo lambarrada platelet, waxaa jira natiijooyin wanaagsan oo isticmaalka PRP loogu talagalay hagaajinta unugyada muruqyada.
Go'doominta platelet-ka waxaa lagula talinayaa in lagu beddelo citrate sodium-ka caadiga ah ee Acid Citrate Dextrose (ACD-A) maadaama habka go'doominta uu u baahan yahay tallaabooyin badan oo dhaqid ah.Platelets waxay aad u deggan yihiin 37C inta lagu jiro wareegitaanka, laakiin ku wareejinta heerkulka qolka (25 C) sidoo kale si fiican ayey u shaqeysaa.Yaraynta pH iyada oo loo marayo ACD-A (waxay ku dhowdahay 6.5) waxay ka caawisaa inay wiiqdo firfircoonida raadadka trombobin ee haraaga ee tuubooyinka platelet, waxayna gacan ka geysataa dayactirka guud ee qaab-dhismeedka platelet-ka iyadoo shaqada u wareejinaysa mid yar.Caadi ahaan waxaad u baahan tahay inaad dib ugu celiso platelets si aad u soo ceshato shaqeyntooda Tyrode Buffer (pH 7.4).ACD waxay leedahay faa'iidooyin badan marka ay timaado ilaalinta platelets
Markii ACD la isticmaalay, natiijadu waxay muujisay wax-soo-saarka platelet oo sarreeya ee dhiigga guud.Si kastaba ha ahaatee, isticmaalka EDTA ayaa sidoo kale dhiirigelisay kobaca celceliska mugga platelet ka dib markii tillaabooyinka centrifugation dhiiga la fuliyay si loo helo PRP.Marka xigta, isticmaalka ACD waxay keentay kororka unugyada stromal mesenchymal.Sidaa darteed, waxaa la soo gabagabeeyey in daawada xinjirowga lidka ku ah oo ay ku jirto ACD-A ay door weyn ka ciyaaraan diyaarinta PRP oo ay gacan ka geystaan wanaajinta habka ilaa xad.
