Tubbada Kala soocida Jeelka Kelida ah ee Unug-Muuqaal—Tuubada CPT

Tuubbada Kala soocida Jeelka Keliga ah ee Muclear—Tuubada CPT Sawirka leh

Sharaxaad Gaaban:

Waxaa loo isticmaalaa in monocytes laga saaro dhiigga oo dhan.

Waxaa inta badan loo isticmaalaa ogaanshaha shaqada difaaca ee lymphocyte sida HLA, ogaanshaha hidda-socodka leukemia-ka haraaga iyo daaweynta unugyada difaaca jirka.

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Faahfaahinta Alaabta

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Waa maxay CPT Tube

Tuubbada kala soocida unugga-muruqa ee kelida ah (tuubada CPT) ayaa lagu daraa hypaque, xinjirowga lidka ku ah iyo jel kala soocida.Lymphocytes iyo unugyada mononuclear si fudud ayaa looga go'doomi karaa dhiigga oo dhan iyadoo la isticmaalayo hal-tallaabo centrifugation marka la isticmaalayo jel kala soocida unugyada gaarka ah.Waxaa inta badan loo isticmaalaa ogaanshaha shaqada difaaca ee lymphocyte, HLA ama ogaanshaha hiddasidaha leukemia ee haraaga ah iyo daaweynta unugyada difaaca jirka.Waxay bixisaa hab caadi ah oo hal-tallaabo ah oo laga soo saaro monocytes-ka loogu talagalay diyaarinta muunada ogaanshaha ee bukaan-socodka iyo immunotherapy-ga gacanta.

Shaqada Alaabta

1) Cabbirka: 13*100mm, 16*125mm;

2) Mugga wax lagu daro: 0.1ml, 135usp;

3) Mugga dhiigga: 4ml,8ml;

4) Nolosha shelf: 24 bilood laga bilaabo taariikhda la soo saaray;

5) Kaydinta:Ku kaydi 18-25℃

AlaabtaFaa'iidada

1) hufan, sax ah oo badbaado leh;

2) Iyadoo la dhisay Ficoll Hypaque, xinjirowga lidka ku ah iyo jel kala-soocida, unugyada mononuclear-ka ayaa laga soocay dhiigga oo dhan hal-tallaabo centrifugation.

3) Farsamada kala soocida unugyada saxda ah.

4) Farsamaynta xuubka bionic ee gidaarka gudaha;

5) Heerka soo kabashada monocytes waa in ka badan 90%, daahirsanaantu waa in ka badan 95%, heerka badbaadaduna waa in ka badan 99%

Arrimaha U Baahan Fiiro Gaar ah

Qodobada soo socda waa in la xuso:

1) Marka la samaynayo tijaabada unugyada culturing, fiiro gaar ah u hawlgal aseptic, jeermis dilay reagents (xalka kala soocidda, xal dhaqidda, iwm) iyo qalabka .Hawlgalkan waa in ay ka shaqeeyaan xirfadlayaal si loo dammaanad qaado isku-duubnida hawlgalka.

2) Heerkulka centrifugation caadi ahaan waa heerkulka qolka (2 ~ 25 ℃) .

3) Caadi ahaan marka la kala saarayo unugyada mononuclear (PBMC) iyo Ficoll, tirada unugyada dhiigga cas waa yar tahay, taas oo saameyn karta saxnaanta tijaabada.Markaa lysate waa la isticmaali karaa (qaar waxay u baahan yihiin in la nadiifiyo), xakamee wakhtiga lysis kana ilaali inay saamayso waxqabadka unugyada mononuclear.

4) Ka digtoonow dib-u-centrifugation kaas oo labanlaabmi kara qasidda.

Hore: CTAD Detection Tube

Xiga: Tuubada Dhiiga Cad ee Cas