Tube ururinta faaruqinta dhiigga cawlan
Sharaxaad Gaaban:
Potassium oxalate/sodium fluoride koofiyad cawl.Sodium fluoride waa daawada xinjirowga lidka ku ah daciifka ah.Caadi ahaan waxaa lagu daraa oxalate potassium ama sodium etionate.Saamiga waa 1 qayb oo ah fluoride sodium iyo 3 qaybood oo potassium oxalate ah.4mg oo ka mid ah isku dhafkan ayaa ka dhigi kara 1ml oo dhiig ah in aanu xinjirin oo uu joojiyo glycolysis 23 maalmood gudahood.Waa ilaaliye wanaagsan oo loogu talagalay go'aaminta gulukooska dhiigga, loomana isticmaali karo go'aaminta urea habka urease, ama go'aaminta fosfatase alkaline iyo amylase.Lagu taliyay baaritaanka sonkorta dhiigga.
Ujeedooyinka: Gulukoosku waa mid ka mid ah falanqaynta ugu badan ee lagu cabbiro shaybaarrada.Daraasadihii ugu dambeeyay ee ku saabsan xasilloonida gulukooska ayaa xaqiijinaya in tubbada sodium fluoride/potassium oxalate (NaF/KOx) ay ka fog tahay heerka dahabka.Tuubooyinka citrate ayaa loo soo jeediyay inay yihiin nooca tuubada ee la door biday machadyo badan.Greiner waxa uu soo bandhigay tuubo gulukoos gaar ah (Glucomedics) oo ka kooban NaF/KOx, citrate, iyo EDTA si loo yareeyo glycolysis.Ujeeddadu waxay ahayd in la go'aamiyo tuubada ugu habboon qiyaasta gulukoosta saxda ah ee goobta shaybaadhka caadiga ah.
Naqshadeynta iyo hababka: Habka daraasadda ayaa ku lug lahaa saddex tijaabo: (a) isbarbardhigga ka qaybqaataha iyadoo la isticmaalayo balaasmaha lithium heparin sida muunada isbarbardhigga;(b) daraasad degganaansho (0, 1, 2 iyo 4 h);iyo (c) mugga buuxinta ugu yar ee citrate iyo tuubooyinka Glucomedics.
Natiijooyinka: Daraasadda isbarbardhigga bukaanka ee plasma heparin ee lithium waxay muujisay in EDTA, NaF/KOx, iyo labadaba citrate iyo Glucomedics haddii la saxo arrimo la isku daro ay keeneen natiijooyin la aqbali karo.Daraasadda xasilloonida ilaa 4 saacadood waxay muujisay in tuubada Glucomedics ay aad waxtar ugu leedahay ka hortagga isbeddelka muhiimka ah ee kiliinikada ee fiirsashada gulukooska heerkulka qolka.Labada citrate iyo Glucomedics waxay u baahan yihiin in lagu buuxiyo 0.5 mL ee mugga buuxinta lagu taliyey ee natiijooyinka la aqbali karo.
Gabagabo: Tuubada Glucomedics ayaa ah tan ugu habboon in la yareeyo glycolysis.Horumarin dheeraad ah oo ku saabsan (isticmaalka qodobka saxda ah ee qasilaha ah iyo ku darida kala-soocida jel) ayaa ka dhigi doonta tuubadan bartilmaameedka qiyaasta ugu saxsan, ogaanshaha ugu fiican iyo go'aamada daryeelka bukaanka.
